Strikes by ScotRail workers during COP26 are set to go ahead as planned, the RMT union has confirmed.
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Industrial action over pay and conditions from 1 to 12 November would coincide with the climate change summit in Glasgow.
The RMT said last-minute talks had failed to reach agreement.
ScotRail said it was disappointed a "very good offer" had been rejected while Transport Scotland said RMT members should have been balloted.
RMT general secretary Mick Lynch said the decision to go ahead with industrial action was made after the train company "failed to get serious" in talks with the union.
The dispute is linked to an ongoing row over pay which has affected Sunday services across Scotland in recent months.
Thousands of delegates from across the globe, including world leaders such as US president Joe Biden, will be in Scotland for the summit next month.
Mr Lynch said: "There was a golden opportunity for ScotRail to make serious progress in talks today, but instead they offered nothing of any consequence and as a result our action throughout COP26 goes ahead as planned.
"There is still time to avoid the chaos of a transport shutdown during COP26 if the key players get back with some serious proposals."
He said the union remained open to pay rise discussions with ScotRail, but "the ball is firmly in their court".
Earlier, Mr Lynch said he had written to the first minister "calling on her to intervene as a matter of urgency to bring about a fair resolution" to the dispute, and accused rail bosses of having "dragged their heels over further talks" with just weeks until the summit.